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Each gene set contained four constructs with distinct target sequences, all of which were packaged for viral production and infection, and tested for target knockdown.
The AMG recognition properties of all the individual sequences were then tested for target specificity in a high-throughput pull-down assay using 96-well streptavidin-coated plates.
Our approach to allow detection of multiple targets in a single 3' UTR was to fragment the database sequences into segments no longer than 50 nt, each of which is tested for target sites.
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Particularly, RGD-conjugated gold nanoparticles were reported and tested for targeting efficiency and radiosensitization as well, but in vitro evaluation was rarely reported [23].
When tested for target-specific cytokine secretion, T cell lines from all EBV-positive donors recognized autologous and allogeneic LCLs in an MHCII-restricted fashion.
Therefore, to test for target redundancy within the miR-30 family, we used a custom-designed probe-set to simultaneously inhibit all 5 members of the family: miR-30a, b, c, d and e.
Upon testing for target RQ value compliance per sample with each endogenous control, TPB yielded the most unstable results and was thus not included in the final assessment of RQ values.
Another decision to be made for a WAF test is the types of association relationships to be tested for the target words selected.
Each of the three heterogeneous mixtures was tested for each target organism after a background reading was taken for the species-specific CNT microfluidic chip.
MF tested for miRNA target site enrichments.
The instruments have been validated and tested for the target group of respondents.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com