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Opa loop-specific antibodies were tested for specificity, surface-binding, in vitro activity against N. gonorrhoeae and the capacity to protect female mice from experimental genital tract infection when delivered topically.
IgG samples with the highest growth-inhibitory activity were tested for specificity of inhibition by reversal of the GIA using specific antigen.
All anti-sPLA2 antibodies were tested for specificity towards the various sPLA2s and were shown to be highly specific for each human sPLA2 (Haas et al, 2005).
We tested for specificity, accuracy, limit of detection (LoD), robustness and precision.
Of the numerous Campylobacter assays that do exist, to our knowledge none has been empirically tested for specificity using high-throughput sequencing.
The assay was tested for specificity and optimized using genomic DNA extracted from identified male worms of Strongylus and cyathostomin species.
The multiplex primers and probes were tested for specificity against 31 pathogenic E. coli strains, nine nonpathogenic E. coli and non-E.coli enteric and environmental bacterial strains.
However, more research is needed to identify promising viral markers for livestock because of cross-reactions that were observed among livestock species or the limited number of samples tested for specificity.
The primer combination was tested for specificity on selected pure cultures, and by comparative sequence analyses of randomly selected clones of a clone library generated with these primers from soil DNA extracts.
For PCR-ELISA, oligonucleotide probes based on 16S rDNA sequences were designed and tested for specificity and sensitivity with nine different bifidobacterial species followed by analysis of faecal samples.
Candidate probes were tested for specificity against the aforementioned R. conorii genome using Olicheck [42].
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