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Different explant types from crambe seedlings were tested for shoot regeneration using different hormone-combinations. Cotyledonary nodes on basic medium with 0.5 μM NAA and 2.2 μM BAP gave the highest regeneration percentages.
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MS (Murashige and Skoog, [1962]), WPM - Woody plant medium (Lloyd and McCown [1981]); B5 (Gamborg et al. [1968]); N6 (Chu et al. [1975]) and White ([1963]) without plant growth regulators (PGRs) were tested for initiation of shoots.
Shoot apices from each of the above cryopreservation steps were propagated on standard medium for fifteen days and tested for survival rates by counting shoot apices that exhibited visible growth (F and P) or pertained greenish and swollen (D and LN).
"The government was careless not to pay to have the badgers tested for TB when they were shot last year.
After a twelve-week period, hygromycin-resistant shoots were tested for transgene integration and expression by PCR and qRT-PCR analysis, respectively.
Other residents requested that the site, a former shooting range, be tested for residual lead from bullets.
Residuals of the data (shoot DW, N and P content in shoot and RLC %) were tested for normality using the Shapiro Wilk test.
There were no significant differences in the root-to-shoot sulfate transfer among all genotypes tested, for both Pi treatments.
Trial and error testing for shooting down drones can reveal a lot about what makes very small aircraft harder or easier to find and kill.
The inclusion of root mass in analyses of shoot mass to test for size-dependent variation in root : shoot allocation [see Supporting Information— Table S2 ] showed that the allometric slope varied between grasses and forbs [significant interaction FG × log root mass)]: forbs had a steeper allometric slope than grasses (αforbs = 0.74 vs. αgrasses = 0.51).
Rooted in vitro seedlings of eight-weeks-old were taken for testing the shoot forming potential of explant types, such as leaves, petioles, stems and axillary buds.
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