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Mesenchymal stem cells (MSC) have been isolated from a variety of tissues and tested for differentiation into different cell lineages.
We then tested for differentiation between origins depending on the rearing environment after three months (T3).
We tested for differentiation between Atlantic morphotypes and between geographic regions within morphotypes (e.g. North Atlantic versus South Atlantic) using φST based on pairwise differences.
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In order to test for differentiation in notochord growth trajectories, a generalized additive model (GAM) [ 61] was used as no a priori parametric model of growth trajectories were available under the prevailing experimental conditions.
We used six circadian clock gene markers and one spawning time QTL-linked marker to test for differentiation among the fall, spring, and FRS migratory groups: OtsClock1b, Cryptochrome2b.2, Cryptochrome2b.3, Cryptochrome3, OmyFbxw11, Omy1009UW, and Ots515NWFSC.
Therefore, the groups of samples obtained for each sampling site were tested for genetic differentiation - based on haplotypes at PIP genes - and lumped together when differentiation could not be detected.
The effect of a bioactive molecule miR-590-5p miR-590-5p miR-590-5ptested for osteoblast differentiation at the cellular and molecular levels using mouse mesenchymal stem cells (C3H10T1/2).
Furthermore, we also tested for possible differentiation in parasite communities infecting the species in sympatry, which we hypothesized could be a driver of divergent evolution at the MHC.
MSCs were cultured at low confluence in IMDM, 10% FCS, 10 ng/ml PDGF-BB (PeProtechEC, London,UK) and were tested for multilineage differentiation into adipocytes, chondrocytes and osteoblasts [36], [37].
Here, we tested for significant differentiation associated with habitat type at different geographical scales.
Supplemental Figure S1 summarizes the results obtained for other miRNA pairs tested for MCI differentiation from age-matched control.
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