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All plasmids were sequence verified and tested for correct expression as described below.
Clones were tested for correct insert sequence and orientation using the M13 forward and reverse primers.
All constructs were sequence verified and tested for correct expression.
BACs were tested for correct chromosomal location by FISH on metaphase spreads together with the respective chromosomal libraries.
The construct was tested for correct sequence, linearized with BlpI, and used for transforming S. pombe strains by homologous recombination.
BACs were first tested for correct genomic localization by FISH on metaphase chromosome spreads of the respective cell line.
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For the purposes of quality assurance, eCSedTrend is provided with a data set that can be used to test for correct software behaviour, especially after specific adaptations and upgrades.
All of the peptides were manually validated by searching a non-deuterated protein sample's MS scan to test for correct m/ z state and to check for the presence of overlapping peptides.
Sampling bias can be predicted, tested for, and corrected [ 17].
All devices should be tested for leaks and correct flow characteristics before use in experiments.
The ROTEM® device was tested regularly for correct function using quality control serum (ROTROL®; Pentapharm GmbH).
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