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Through the use of host bloodmeal source identification, we demonstrated that I. ricinus larvae had ample opportunity to acquire A. phagocytophilum infection from voles (over half the questing I. ricinus nymphs we tested fed on voles as larvae).
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E2 strain productivity was also tested in fed-batch cultures, in conditions optimized for biomass productivity by providing excess CO2 and enriching the media for nitrogen, phosphorus and iron (see " Methods" for details).
This month, officials testing hay fed to cattle at a ranch in Minamisoma detected radioactive cesium 250 times above Japan's official limit.
In a within-subjects repeated measures design, 15 subjects (nine male; six female) aged 28.6 ± 4.5 years with initial body weight (BW) 74.7 ± 4.9 kg and body mass index (BMI) 25.3 ± 1.4 kg/m2 were randomized and tested at baseline (FED) and Post Deprivation (FASTED) for nasal chemosensory performance (Sniffin' Sticks) and food palatability (visual analogue scale).
We tested both lifespan-fed wandering L3 and 12-hour control and high-sugar-fed mid-L3 staged larvae to allow us to compare the effects of diet in both feeding and post-feeding larvae (supplementary material Fig. S2).
The feed profile design was tested on a fed-batch process simulation.
The efficiency of the method has been tested in a fed-batch fermentation having the goal to increase the biomass production.
The productivity of this optimized strain was then tested in a fed-batch stirred tank bioreactor, where final product concentrations of up to 86.2 mg/L with a yield of 0.0318 g- 2,3-BDO) /g- 2,3-BDO obtained under O2-limited conditions.
When tested with CL-fed chicken muscle tissues, the protein microarray assay had higher sensitivity (0.9 ng/g) than the ci-ELISA (0.1 ng/g) for detection of CL residues.
It has been tested on a simulated fed-batch production scale fermenter.
In order to improve the level of secreted ATH35L, we designed a series of novel signal peptides by swapping individual domains of modifying dsbA and pelB signal peptides and tested them in a fed-batch fermentation process.
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