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Among 13 adult and 13 fetal tissues tested, expression of the six BORIS subfamilies was detected only in adult testis and in embryonic ovary, but the relative levels of isoform expression were reproducibly different in the two tissues (Fig. 4A,B).
We also tested expression of the protein by Western blot analyses.
We next tested expression of the established negative regulators SEF and DUSP1.
We next tested expression of the transcription factor GATA-3 at steady state and in infected mice, which has recently been recognized to control both Foxp3 expression in vivo [ 37] and Foxp3+ T-cell fate and function [ 38].
Of the tumour tissues tested, expression of the 10 kDa S100A4 protein was strongly detectable in MDA/EGFP tumour tissues but was decreased to almost undetectable levels in all MDA/RLN2 xenograft tissues indicating the down-regulation of S100A4 during long-term exposure to RLN2 to be also effective in vivo.
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Primers used for testing expression of the U2, U4 and U6 snRNA candidates are listed below: Thanks to Errol Kwan from MicroAquatech for supplying Giardia cell culture.
To test expression of the Bcr1-regulated gene ALS3 in an oral model of infection, we quantified expression by real-time RT-PCR after 24 h growth on the 3D model of the human oral mucosa.
In contrast to our work with proteins from P. aeruginosa, most of the studies tested expression of TM proteins from E. coli or from a thermophile.
In contrast to all other genes tested, expression of FRK1 was synergistically increased by the combined SA and ozone treatment.
We also tested expression levels of the five most significantly modified miRNAs using quantitative RT-PCR (qRT-PCR).
The test expressions of the mutants were analyzed following lysis and centrifugation.
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