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We tested expression of Turbo, tdTomato, mCherry, mKat and mPlum expressed from Phsp60 or Psmyc.
We further tested expression of GH receptor in protein lysates of testes (Figure 8B), ovaries, thymus, and adrenal glands (data not shown).
We tested expression of Gnat1 and Gnat2 as correlates for the presence of rods and cones, respectively.
We tested expression of RA3 in multiple Escherichia coli (E. coli) through Isopropyl β-D-1-thiogalactopyranoside induction and variating temperatures and growth time.
In addition to PGCs, we tested expression of retrotransposons in 3 days post partum (dpp) spermatogonia.
We tested expression of kinin receptors by flow cytometry and migratory capacity of circulating monocytes and progenitor cells towards bradykinin (BK) in transwell migration assays.
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Primers used for testing expression of the U2, U4 and U6 snRNA candidates are listed below: Thanks to Errol Kwan from MicroAquatech for supplying Giardia cell culture.
Therefore, we sought to test expression of IK2 in puf2 sporozoites, in comparison to WT and puf1 sporozoites, using RT-qPCR.
Given that Scarb1 was an obvious candidate, real-time PCR was performed using liver tissue to test expression of Scarb1 between HLB398 mutant mice and B6 controls.
Also, we did not analyze other immunohistochemical subtypes by testing expression of MUC1, MUC2, etc.
As proof of principle we demonstrate the functionality and efficiency of this system by testing expression of several cDNA and shRNA sequences in a number of cell lines.
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