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I just wanted to be him, the way he graced the pitch" – his desire to play professional football was tested by serial early rejection, most citing his size and a perceived lack of fitness.
Strain GS_P GAP -tapA was obtained and tested by serial dilution spotting on YPD plates supplemented with different concentration of lovastatin.
PCR reaction efficiencies for all primer pairs were tested by serial template dilution, and were between 90% and 110%.
Formaldehyde tolerance was tested by serial dilution and spotting on SD (-Ura) plates containing the indicated concentration of formaldehyde.
If the cancer stem cells are indeed rare (Adams and Strasser 2008), one possibility is that there has not been enough time in most neoplasms to select for an increased frequency of cancer stem cells, an hypothesis that could be tested by serial passage of neoplastic cells through immune compromised mice.
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Growth was tested by spotting serial dilutions at an estimated 105 100 cells/spot, left to right on parallel synthetic complete (SC) agar medium with or without added AAAs (tryptophan, tyrosine, phenylalanine) and incubating the plates at 30 °C for 3 days.
The quantitative parameters of phage-based qPCR were first tested by using serial ten-fold dilutions of DNA purified from φA1122 and L-413C.
Sensitivity of WT, SA and SE cells to benomyl was tested by spotting serial dilutions of cells either on normal YPD plates (control) or on YPD plates containing benomyl, and examining cell growth at 30°C (Figure 1B).
The specificity of each primer pair and efficiency of the amplification were tested by assaying serial dilutions of the cDNA.
The specificity of each primer pair as well as the efficiency of the amplification step was tested by assaying serial dilutions of cDNA.
The growth properties of each mutant were tested by spotting serial dilutions of galactose-grown cells on media containing fructose or glucose as the carbon source.
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