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The spiked sample was also tested by diluting it to determine if it dilutes parallel to the standard curve.
The interaction of DAF or fluorescein sodium with proteins was also tested by diluting the standards in PBS containing 10% fetal bovine serum (FBS).
The analytic sensitivity of the reaction was tested by diluting the toxin in Hepes reaction buffer to 100, 10, 1, 0.1, and 0.01 MLD50.01.
The influence of the type of sample on the PCR reaction was tested by diluting the standard 8E5 cells in HIV seronegative plasma from a blood donor before DNA extraction.
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Six intact sandal charms and six intact heart-shaped charms were tested by dilute HCl extraction for 6, 24, 48, and 96 hr.
The Q-PCR system was tested by serially diluting samples to confirm the linear relationship between Ct values and the log of sample mRNA concentration (data not shown).
The whole system was tested by using diluted rubber latex with different TSCs at room temperature (25 °C) and the results of standard oven drying method and this study were found with the accuracy and reproducibility at the level of less than 1%.
But cyclists can dupe this test by diluting their blood with saline.
Work solutions of 5 and 20 μM of MCLR were prepared immediately before testing, by diluting the stock solution in cell culture medium or saline solution.
The latter possibility was tested by a dilute-and-shoot approach.> -wrap-foot> hydrolysisisH shortenedned hydrolysis aMean of two measurements To test whether BMPEA may be eliminated in a free form, excretion samples were analyzed by using a dilute-and-shoot method.
Nitrocellulose was then probed overnight with the anti-oleosin polyclonal rabbit antibody produced and tested by the laboratory (diluted 1∶10 in TBS with 0.1% Tween 20).
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