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Target miRNA expression was normalized between different samples based on the values determined for let-7a miRNA expression which was found to be routinely consistently expressed in all of the samples tested at the same Ct value (no differential expression between samples).
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Whereas all animals were trained and tested at the same ZT, the fact that jet-lagged animals did not entrain to the LD cycle resulted in an incongruence between CT and ZT in jet-lagged hamsters.
And everything has to be tested at the same time.
For the purposes of this test, we used the same CT scan of the TPC from the same specimen of Tursiops truncatus.
Most optimal test estimates for the real-time PCR assay were at a Ct-value cut-off of 37; 0.93 [95% posterior probability interval (PPI) 0.60-0.99] for Se and 0.95 [95% PPI 0.95-0.99] for Sp. At the same Ct-value cut-off, Se and Sp for BC were 0.83 [95% PPI 0.66-0.99] and 0.97 [95% PPI 0.91-0.99] respectively.
Because we are comparing the normalized CT responses before and after TRPV1t modulators in the same CT preparation, paired t-test was used to evaluate statistical significance.
Radiotherapy-planning CT scans were performed at the Department of Radiation Oncology following the imaging protocols described above with the same CT scanners, contrast medium, scanning areas, resolutions and calculation protocols.
The same CT scanning and reconstruction parameters should be used at LDCT follow-up studies.
Briefly, the ΔCT value was obtained by subtracting the 18s rRNA CT value from the CT value of the gene tested in the same sample.
At the same time, CT and TEP scans detected the presence of multiple asymptomatic liver and lung metastases.
The delta CT values of GAPDH were used to normalise all other genes tested from the same cDNA aliquot.
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