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We therefore tested a role for PERK activation in mediating starvation-induced mRNA translation.
We tested a role for Zfp706 in the exit from pluripotency by siRNA knockdown and observed interference with Rex1GFP downregulation.
We therefore tested a role for VPA in reducing inositol levels following acute treatment (9 minutes; 0.5 mM; Fig. 5), in a similar manner to phosphoinositide measurement experiments.
To improve rhythms in old flies, we manipulated environmental conditions, which can have direct effects on behavior, and also tested a role for molecules that act downstream of the clock.
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We began by testing a role for TACC3 in interphase cell migration.
Looking forward we are greatly interested in directly testing a role for NEUROD2 in the cortical migration process.
To test a role of Myo5 in CSV motility further, we investigated G3Mcs1 localization and motility in a myo5-null mutant (ΔMyo5).
To first test a role for the proteasome, we treated transfected cells with MG-132, a proteasome inhibitor, prior to oxidative stress treatment.
To test a role of AIP1 together with LHP1 in flower development, the expression of genes epigenetically repressed by LHP1, known as floral development target genes such as Flower Locus T (FT), Agamous (AG) and APETALLA 3 (AP3) [ 50], was analyzed.
We also tested a reinforcing role of oambP-GAL4 neurons by pairing their activation, using UAS- dTrpA1, with odor presentation.
However, genes from the 3p25.3 and 15q21.3 regions have not been identified or systematically tested for a role in AE.
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