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A Tracey-Wisdom test with default parameter settings was performed to determine the significance of axes using the twstats program.
Enrichment analysis was performed using Fisher's exact test with default parameters (significance threshold of 0.05) available in Blast2GO to identify significantly enriched GO terms.
Tests for deviations from Hardy-Weinberg equilibrium (HWE) were performed across all loci by GENEPOP 3.4 [ 16] applying the exact test with default settings of the Markov chain Monte Carlo methodology.
SSR loci were also tested for linkage disequilibrium for each pair of loci in each population using the genotypic linkage disequilibrium test with default Markov chain parameters in Genepop.
In an independent benchmarking test with default cutoffs [ 54], IUPRED achieved 59.5% sensitivity with 95.6% specificity; the sensitivity of VSL2 reached 75.5%, but its specificity was 79.4% (the sensitivity of VSL2B was shown to be approximately 5% lower compared with VSL2 at a similar specificity [ 52]).
Enrichment analysis for statistical significance of GO annotation between two groups of annotated sequences was performed using Fisher's Exact Test with default parameters.
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Next, Fisher's exact test was run with default values (a two-tailed test that removes double IDs, with a false discovery rate (FDR) cut-off of 0.01).
Then, Fisher's exact test was run with default values: a one-tailed test without removing double IDs and 0.05 false discovery rate (FDR) cut-off.
The differentiation coefficients between each pair of P. infestans populations (FST) were estimated according to Weir and Cockerham (1984) using genepop 4.0.7 (Raymond and Rousset 1995), and their statistical significance was tested with the exact G-test (with default Markov chain parameters) implemented in genepop.
The method was tested with default settings.
Prophage Finder was first tested with default parameters and second with a more strict E value threshold (1e-3).
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