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For comparison of categorical variables, significance testing was done by χ2 test with by 2-sided Fisher exact test as appropriate.
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Echocardiographic parameters were compared between tertiles of NT-proBNP using the Jonckheere-Terpstra test or the Chi-squared test with linear-by-linear association.
ErbB2 status is determined by using the Hercep test with confirmation by FISH.
Comparisons between groups were done by the Kruskal-Wallis test with corrections by Bonferroni for multiple testing.
Plasma samples were tested for herpes simplex type 2 (HSV-2) antibodies and for syphilis by Rapid Plasma Reagin test with confirmation by a Treponema pallidum-specific test.
Comparisons between groups were done by the Mann-Whitney U test with corrections by Bonferroni for multiple testing.
Survival curves were compared using the log-rank test, with stratification by site for comparisons that pooled data across sites.
Comparison of durations of antibiotic therapy will be done using the Mann-Whitney test with stratification by centre.
The diagnostic accuracy of a clinical test is typically evaluated by comparing the classification of individuals by the test with their classification by some diagnostic gold standard.
Statistical analysis was by Mann-Whitney U test, with significance determined by P < 0.05.
Data was analyzed by t test with p < 0.005 followed by ratio analysis (minimum 2 fold change).
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