After 6 h in a moist chamber, the test circles were stained with rose bengal, and spore germination and germ tube length were determined under a microscope equipped with a vertical illuminator (Ko,1971).
In brief, spleen cells from syngeneic and test mice were stained with 0.3 and 3 µM, respectively, of the dye 5,6-carboxyfluorescein diacetate succinimidyl ester (CFSE; Molecular Probes).
Because this was a pilot study, the HER2 testing TMAs were stained with three different antibodies and mono color SISH and dual color SISH in order to ascertain optimal HER2 testing methods for the purpose of this TMA evaluation.
For the smear test, fixed preparations were stained with auramine and visualized under a fluorescence microscope (at × 400 magnification).
To test viability, parasites were stained in parallel with the LIVE/DEAD Assay (Invitrogen AG, Switzerland) using a 1 : 1000 dilution.
In the long term viability test, PC9 cells were stained with DiI red fluorescence cell tracker before hydrogel encapsulation and seeding in a 3D μFCA with initial cell seeding density of 60 million/mL.
Following incubation with test compounds, spheroids were stained with a mixture of three dyes: 2 μM calcein AM, 3 μM of EthD-1, and 33 μM Hoechst 33342 (Life Technologies, Carlsbad, CA).
To test this hypothesis, flagella were stained in both the wild-type and Δ recA cells.
To test this interpretation, mutant embryos were stained for Caspase-3, a marker of apoptosis (Srinivasan et al., 1998).
Note that when random colonies were tested, cells in all clones were stained (~100 % positive) with anti-BTAK (anti-tumor) antibody (data not shown).
