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Analysis of variance by Duncan's test and Pearson's product-moment correlation coefficient test were determined using SPSS version 10.0 software (SPSS Institute, Inc., Chicago, IL, USA).
Cut-points for maximising the sensitivity and specificity for each test were determined using the Youden index.
Survival rates of two Anisakis species in acid tolerance test were determined using the Kaplan-Meier survival method and differences between groups were identified using log-rank analysis.
Continuous variables that approximately adhered to a normal distribution according to the D'Agostino k-squared test were determined using an analysis of variance.
Using statistical software (SPSS Version 20.0, SPSS Inc., Chicago, IL, USA), mean between-group differences in subject characteristics, pulmonary function data, and endpoints of the maximal cardiopulmonary exercise test were determined using independent t-tests.
All summary statistics, such as allele number per locus, effective allele number per locus, Shannon's information index, genotype number per locus, gene diversity, PIC values, observed homozygosity, genetic distance, and Fst test, were determined using POPGENE version 1.32 and PowerMarker version 3.25 (Liu and Muse 2005).
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This test was determined using undisturbed samples following the standard method described by the NF P94-071-2 normAFNOR 1999(AFNOR 1999).
The pore volume injected (PVI) versus the pressure drop data for mobility control test was determined using core flood experiments.
The statistical significance of the correct number of judgments in the triangle test was determined using the tables described in [36].
The sensitivity of the test was determined using optical microscopy (OM) as the gold standard [ 23].
Successful toleration of the DSA test was determined using the Consensus Conference on Weaning criteria described above.
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