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Here, again, it would be instructive to test unwinding with the nucleotide triphosphates.
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WASHINGTON — Brutal winter weather contributed to a downturn in job growth and other economic data, raising questions about the strength of the recovery and testing the Federal Reserve's resolve in unwinding its key bond-buying stimulus program.
Nicking was tested by SDS PAGE, using the single oligonucleotide, ICRII-part, and unwinding the 20 bp-F junction by the Cy3-Dabcyl assay.
Millions of Americans visit casinos to unwind and test their luck against the hands of fate, but lurking in the shadows is a gamble few would contemplate before they stepped inside a casino's doors.
This prompted us to test its ability to unwind recombination intermediates, such as HJs or D-loop structures.
Several substrates (Table 1) that would generate a 30bp duplex product with varying length overhangs were tested in the absence of ATP and Mg2+ to eliminate complicating effects of unwinding.
DD31′ is a gapped DNA substrate slightly modified from DD31 by repositioning the Cy3 fluorophore, and is used to test whether the presence of a Cy3 fluorophoe at the ss dsDNA junction would affect Pif1 unwinding.
To determine if any of the holoenzymes could activate T antigen, we tested the ability of the heterodimeric AC and two heterotrimeric ABC forms to stimulate T-antigen function in unwinding the origin of SV40 DNA replication.
He's unwinding.
These factors are unwinding.
Mr. Barber was also unwinding.
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