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However, validated immunoassay kits to test this specificity have not been commercially available.
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To test whether this specificity is recapitulated in eukaryotic cells, we examined the responsiveness of the T3N-LasR protein to synthetic autoinducers that differed at position 3 on the acyl chain.
Like for the cobas® 4800 BRAF V600 test this p.V600E specificity constitutes the major limitation of the IHC for routine diagnostics as a single test.
To further test the specificity of this interaction, we performed different in vitro assays (Figure 2).
To test the specificity of this model for chronic versus acute colitis, acute serum was input into the model.
To test the specificity of this Cre-Lox system in vitro, MDC-Cre and MDC-Lox cells were mixed (1∶1) in differentiation medium to promote muscle cell fusion.
In order to test the specificity of this PB signature of human radiation response, we next tested its accuracy in predicting the status of patients who had undergone chemotherapy treatment alone.
In order to test the specificity of this antibody, the effects of the IL-4 neutralising antibody on cell growth in the presence of exogenous IL-4 were examined.
To test the specificity of this sensor, we combined the Far1DS-NLS-RFP and Ste7DS-NLS-YFP sensors in the same cells and quantified their responses in wild-type (WT) and MAPK deletion strains (Fig. 6c and d).
To test the specificity of this apparent inhibitory effect of Hif-1α on β-catenin activity, we overexpressed Hif-1α by eliminating the expression of Vhl, and as expected this genetic manipulation impaired the ability of PTH to increase the expression of Axin2.
To test the specificity of this antibody to methylated FoxO3, we performed an in vitro methylation assay with wild type (WT) FoxO3 or the FoxO3 K271R mutant as substrates, and either WT Set9 or a methyltransferase-deficient version of Set9 (H297as as the enzyme [ 66].
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com