Exact(7)
In an attempt to test this point further, we introduced the L57P mutation into Brk1 as a way to impair the formation of the C-terminal coiled-coil.
To test this point, infectivity of intracellular infectious particles was analyzed following lysis of HCV-JFH1-infected knockdown cells by repetitive freeze and thaw.
Future experiments are warranted to test this point directly.
We carried out three more experiments to test this point further.
To further test this point, we obtained preliminary methylation data for the CpG sites reported in Tables 1 and 2, which have been normalized using the pipeline described in Touleimat and Tost (19).
Another way to test this point is to induce VCP aggregation in a proteasome-independent way using protein homo- or hetero-dimerization systems (i.e. FKBP-FRB) to see whether a similar effect is obtained.
Similar(50)
We have tested this point by changing the number of patches in our system to six and run the simulations again (data not shown).
We also tested this point in the revised manuscript (we kindly refer the reviewers to responses #1 and #3c).
We are very grateful for this comment because we actually tested this point early on in these studies, but neglected to report the findings.
As opposed to the conventional idea that the whole DR complex lies in the midbrain, we thus confirm that only this small component lies rostral to the MHB, having now tested this point with the specific Otx2 midbrain marker.
The operators ought to have cancelled the test at this point, and indeed they proposed doing so, but a supervisor overruled them.
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