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To confirm whether this suppression directly affected hTERT, dual-luciferase reporter assays were conducted to test the potential interaction between hTERT 3′ UTR and these five miRNAs.
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We started by testing the potential interaction between JIL1 and the MSL complex.
We also tested the potential interaction between ROS and reactive nitrogen species (RNS) by inducing arthritis in Ncf1 −/−Nos2 −/− mice.
We also tested the potential interaction between O3-8hr and temperatures variables, and we introduced in the models a variable taking into account humidity levels (maximum daily relative humidity).
Finally, motivated by the study by Schnittker [ 17], our study also aimed at testing the potential interaction between income and education.
A test for the potential interaction between Akt and the benefit from treatment was performed by a Cox model including the covariates Akt, treatment, and the interaction term treatment × Akt.
We tested the potential interactions between each component of the metabolic syndrome and apoE genotype (presence of at least one versus no ɛ4 allele) on the risk of incident dementia.
To test this hypothesis, we investigated the potential interaction of PHY906 and CPT-11 on intestinal damage and antitumor activity in mice with or without depletion of intestinal bacteria.
The potential interaction between T3/TSH and menopausal status was tested by adding an interaction term in the Cox analysis.
Note too that in the random effects model, where country-level variables are included (model 2), the association remains the same, strengthening the belief that the random effects models are correctly specified to test the potential of interaction effects.
To test the direct interaction of miRNAs with the 3'UTR, we developed a PCR assay to test the potential binding interactions of the miRNAs with the 3'UTR and to validate the target sites in silico.
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com