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To test the linkage of reproductive traits and sensory response systems of social behavior, we measured sucrose responsiveness with the proboscis extension response (PER) assay and quantified ovary size and vitellogenin (yolk precursor) gene expression in 6 7-day-old bees by counting ovarioles (ovary filaments) and by using semiquantitative real time RT-PCR.
We calculated the LOD score to test the linkage of the RBPs inferred activities at each locus.
Arlequin 3.11 (Excoffier et al., 2005) was used to test the linkage among microsatellite loci, to test for Hardy Weinberg equilibrium (HWE), and to calculate polymorphism.
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We tested the linkage of lin-11 (sy5336 ) with several phenotypic markers that were assigned to various chromosomes.
Traditionally, an eQTL study tests the linkage between all genes' expression and all loci, adding up to millions of single statistical tests.
To address this issue, we tested the linkage disequilibrium (LD) between the genomic interval of each of our identified modules and the entire genome.
Most rely on an exhaustive, genome scale search that considers all possible locus gene pairs and tests the linkage between each locus and gene.
For Chr8, we also tested the linkage to resistance of three of the four identified regions, in 48 selected strongly resistant F4 individuals of the cross SICS/SRB that had survived at a high dose of 0.8 mg L-1.
The D' test measures the linkage disequilibrium [ 39, 13, 40 ] which is the non-random association calculation of two alleles at two loci.
We investigated clusters containing at least one Patient Administration system (PAS) record, a clinically and epidemiologically important group which represents an important test of the linkage system, as it includes inpatient visits and large numbers of laboratory and other records.
To test the genetic linkage between expression of PDK1, HR46, reproductive anatomy, and social foraging, we collected 697 newly emerged backcross workers (see Materials and Methods).
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