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To test the expressions of CB1 and CB2 receptors in BM-MSCs, RT-PCR was performed with primers of the genes of two receptors.
Despite the lack of significance by the repeated measures test, the expressions of these three genes are exquisitely correlated to CoVa knockdown and serve as time-independent markers of electron transport function.
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Both Western blot and immunohistochemical staining analyses were used to test the expression of Hpa in HepG2, SGC-7901, MKN45, MCF-7, SW480, and U2OS cells.
Primer sets used to test the expression of the two forms of CTIP2 were designed using Primer3 Software.
In addition to this peripheral protein, we also decided to test the expression of five intrinsic Arabidopsis membrane proteins.
hIGF2exon6Fw and hIGF2exon7R were used with universal probelibrary 63 to test the expression of P2 P4 derived IGF2 transcripts.
To test the expression of the iron uptake system of the HPI, yersiniabactin production was tested using a GFP-reporter assay [37] [39].
To test the expression of the iron uptake system, yersiniabactin production was tested using a GFP-reporter assay [2], [40], [57].
This observation allowed us to test the expression of Rae-1δ or Rae-1ε transcripts under the control of promoter 2 alone, which we called short transcripts.
Using a 0.3 RPKM threshold, we chose to test the expression of 23 genes in our DU145 samples using RT-PCR, ten of which demonstrated detectable amplification.
We could then test the expression of tidal phenotypes in the absence of circadian competence.
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