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Quantitative RT-PCR (qRT-PCR) was used to test the expression patterns of fungal ESTs from different organisms including rust-infected leaves [ 20].
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To test the expression pattern of the identified genes, we used expression data from Flyatlas (Chintapalli et al., 2007).
To test the expression pattern of Notch 1 and Notch 4 in an inflammatory neovascularization we studied laser-induced CNV in mice eyes.
We performed additional experiments to test the expression pattern of Cre recombinase in our CamKIIα-Cre line in the hippocampus and neocortex by crossing CamKIIα-Cre mice with the Rosa26-loxP-tdTomato-loxP reporter mice.
We tested the expression patterns of these miRNAs to determine if they also were altered in untransformed B cells upon activation.
In general, for about half of the miRNAs tested, the expression patterns observed by Northern analysis were consistent with the abundance of miRNA reads in the libraries.
We re-tested the expression patterns of 22 available S. latifolia ESTs previously described according to Northern blots or Virtual Northerns [ 22- 24] as preferentially expressed in male flowers and/or early stamen (for the list of the ESTs chosen for this study, see Additional file 1: Supplementary table S1).
This implicates a large number of additional proteins, the function of which in the ENS has not been directly tested and the expression patterns of which are not always restricted to the NC.
To determine how OsLIC affects BR signaling in rice, we tested the expression pattern of OsLIC with exogenous BR treatment.
We, therefore, tested the expression pattern of MLC1 by immunohistochemical staining in glioblastoma tissue.
In our study, we have not tested the expression pattern of the two homoeoalleles in tetraploid wheat.
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