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To test the expression levels of 2-MIB associated genes without light, cultures were cultivated in darkness at the temperature of 25°C for 72 h.
Real-time PCR and western blotting were used to test the expression levels of MR-1.
The FPKM (Fragments per Kilobase of transcript per Million mapped reads) value was calculated to test the expression levels of the unigenes.
To test the expression levels of IGF-1n and IGF-1s, tobacco plants were transformed with the chloroplast transformation vector (pLD) containing either the IGF-1s or IGF-1n gene.
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qRT-PCR was carried out to test the expression level of genes around the predicted insertion point.
Using qRT-PCR we aimed to test the expression level of the above genes in individual CCs from 35 and 36 old patients.
Therefore we independently tested the expression levels of a panel of mitotic regulators that were identified as differentially expressed in the H' vs H analysis.
Therefore, we tested the expression levels of these two genes in their most functionally relevant tissues: SALL1 in kidneys and PTEN in the bone marrow.
We tested the expression levels of 12 randomly selected genes by qRT-PCR analysis (six specifically deregulated genes were chosen on each microarray list).
We tested the expression levels of probes that overlapped with endo-siRNA reads.
We first tested the expression levels of canine BRCA2 in mammary gland and mammary tumor samples by qRT-PCR.
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