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qRT-PCR was carried out to test the expression level of genes around the predicted insertion point.
Using qRT-PCR we aimed to test the expression level of the above genes in individual CCs from 35 and 36 old patients.
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To test the expression levels of 2-MIB associated genes without light, cultures were cultivated in darkness at the temperature of 25°C for 72 h.
Real-time PCR and western blotting were used to test the expression levels of MR-1.
The FPKM (Fragments per Kilobase of transcript per Million mapped reads) value was calculated to test the expression levels of the unigenes.
To test the expression levels of IGF-1n and IGF-1s, tobacco plants were transformed with the chloroplast transformation vector (pLD) containing either the IGF-1s or IGF-1n gene.
For testing the expression level of GUS in leaves, fully expanded 4th leaf of each seedling at four-leaf stage was used.
We selected a number of genes that had roles in transcriptional regulation, development and signal transduction and tested the expression level of these genes using quantitative RT-PCR.
Since previous yeast two hybrid results indicated possible interaction of SNAP29 with other members of the EHD family [12], we also tested the expression level and intracellular localization of other EHDs in the absence of SNAP29.
Therefore, we tested the expression level of miR-29a using the hepatoma cell lines which were reported with different metastasis potential as a model, such as MHCC-97H and MHCC-97L [24].
To further confirm that hsf1-R206S, F256S cells had enhanced basal activation of Hsf1 at 25°C, we tested the expression level of numerous known transcriptional targets of Hsf1 in these cells.
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