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However, in the verification test, the expression rate of YER034W showed the lowest correlation among the three genes.
Both Western blot and immunohistochemical staining analyses were used to test the expression of Hpa in HepG2, SGC-7901, MKN45, MCF-7, SW480, and U2OS cells.
The generated mu-IFN-CSP/pET-21b plasmids were transformed into E. coli BL21 (DE3) to test the expression and solubility.
Dual-luciferase reporter assay, real-time quantitative PCR (qRT-PCR) and related phenotypic experiments were used to test the expression of related genes and the therapeutic effects of our devices.
qRT-PCR was carried out to test the expression level of genes around the predicted insertion point.
In addition to this peripheral protein, we also decided to test the expression of five intrinsic Arabidopsis membrane proteins.
Primer sets used to test the expression of the two forms of CTIP2 were designed using Primer3 Software.
hIGF2exon6Fw and hIGF2exon7R were used with universal probelibrary 63 to test the expression of P2 P4 derived IGF2 transcripts.
This observation allowed us to test the expression of Rae-1δ or Rae-1ε transcripts under the control of promoter 2 alone, which we called short transcripts.
To test the expression of the iron uptake system, yersiniabactin production was tested using a GFP-reporter assay [2], [40], [57].
To test the expression of the iron uptake system of the HPI, yersiniabactin production was tested using a GFP-reporter assay [37] [39].
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