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To test the diffusion distance of the protease-digested goods, we added a LasB substrate to the plate that fluoresces once cleaved by LasB and then repeated the competition experiment above with 1 cm colony spacing.
To assess the impact of glycosylation on the lateral diffusion of CX3CL1, we used FRAP to test the diffusion of the dgCX3CL1 variant and found that its diffusion rate was more than 2-fold larger than that of native CX3CL1 (Fig. 4A).
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In addition, I test whether the diffusion of cohabitation is related to second birth differentials by union type.
For antimicrobial susceptibility test, the disc diffusion test was employed using commercially available 10 antimicrobial discs.
This potentially reduces partial volume effects on statistical testing of the diffusion measurements.
In agar diffusion assays, the inhibitory zone diameter produced is the result of the growth of the test organisms and the diffusion of the test agents through the agar, both events occurring simultaneously [ 15] so it then means that any factor which affects the rate of microbial growth or rate of diffusion of the suspected antimicrobial agent under test, will invariably affect the result.
Of the in vitro release tests, the Franz diffusion cell test gave the release profile that most closely resembled the in vivo data.
In almost all tests, the disc diffusion method confirmed the antibiotic resistances predicted by the microarray analysis.
The model was tested by solving the diffusion equation numerically on example structures that differed in architecture.
F-tests showed that the diffusion data fitted the bi-exponential model significantly better than the mono-exponential model (F > 21.4, P < 0.010).
The drug release mechanism differed with the formulation variables, although if the pellets remained intact during the dissolution test, diffusion was the controlling mechanism.
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