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Therefore, in order to simplify our cotransfection assays, we decided to use protoplasts prepared from ttg1 mutant rosette leaves to test the activation of single-repeat R3 MYB genes by the GL3/EGL3-GL1 or GL3/EGL3-WER activator complex.
1.5 hours after the end of the Morris water maze test, the activation of c-fos in the whole population of dentate gyrus neurons was equal in PC3/Tis21-null and WT mice, since no significant difference in number of c-fos+/NeuN+ neurons emerged (Figure 6E).
Specific antibodies were used to test the activation of the MAPK p38, NF- κB and I κB α.
To test the activation of NGF-TrkA downstream pathways, cells were treated with 100 ng/ml β-NGF (#PHG0126; Life Technology) for 15 min in FBS-free medium.
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Auto-activators were identified by testing the activation of GAL1::HIS3 on minimal medium lacking leucine and histidine but containing 20 mM 3-amino-1,2,4-triazole (3-AT) in the absence of any AD-containing vector.
We tested the activation of these channels relative to depolarizing currents activated by elevated extracellular potassium as a calibration standard.
Simulated experiments are used to validate the convoy model, testing the activation of the different behaviors in the decision-making process.
Our experiments that tested the activation of the human megalin promoter by luciferase reporter assay and the EMSAs, which used human megalin probes, were performed with mouse PPARα.
We tested the activation of PI 3-kinase in THP-1 cells stably transformed with a reporter construct specific for the kinase product phosphatidylinositol (3,4,5) triphosphate (PIP3) [53].
In the Casp3-test, the activation of proteases that recognize the DEVD peptide motif (DEVD-ases), especially caspase-3 was detected by measuring a fluorescence signal generated by a fluorogenic dye upon cleavage by caspase-3.
We tested the activation of p-CREB in the ACC of the pain memory model with IF and EMSA.
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