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To put the mouse data on a firmer footing, the National Institute on Aging has set up a program to test substances in three labs simultaneously.
The first crucial step is to select suitable test substances.
The test substances were cypermethrin and doramectin sc.
The highest concentration of test substances was transferred to the first row of the plate.
The removal of these test substances as well as product formation was determined by HPLC analyses.
NDV was used to examine the effect of all test substances in 23 animals.
Tests were conducted in 96-well plates using different concentrations of test substances.
This test, however, requires [14C] labeled test substances, which clearly limits the scope of the test.
Three test substances of varying sample weight were routinely analyzed within each treatment.
Test substances must produce profound skeletal alterations for bone lesions to be detected by this procedure.
Test substances were reconstituted and added to the scaffold prior to commencing the skin incision.
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