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Test substance pellet.
Was the test substance identified?
As the general test principal, the difference between the gas production from the bottles containing the test substance and the bottles without test substance (blank) is taken as the evaluation criterion for assessing the anaerobic degradation of the test substance.
Using a two-phase addition of test substance (rather than three dosings) was deemed a compromise between the test length and the potential to overestimate test substance degradation.
Figure 8 Degradation of test substance # 61 DID in the modified (two-step) testing scheme.
For non toxic compounds, even higher test substance concentrations may be appropriate.
Data on dosing the test substance (static, semi-static, flow through system).
Test substance was diluted with media into the desired concentrations from the stock.
For this, the carbon content of the test substance needs to be determined.
Neither stability nor by-products of the test substance was stated in any of the studies.
Individual test substance degradation values (mean and standard deviation) are shown in Figure 4.
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