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However, the test strain exhibited sensitivity towards chloramphenicol, kanamycin and tobramycin (Table 2).
RPMI 1640 containing 10% (v/v) FCS was infected with the test strain.
(a) ● Escherichia coli ATCC8739 (disinfectant test strain) test; ◯, ATCC8739 control; ▼, ESBL E. coli test; ▽, ESBL E. coli control ◯.
The typical screening involved the cultivation of the test strain on FMZ-agarose medium at 28°C for 7 days.
Test strain of PC was transferred to sterile PDA slants and stored at 4 °C before use.
ESBL E. coli was more resistant than the standard test strain but was still effectively killed within 24 h.
The antimicrobial activity of coating 1 against the standard test strain of E. coli (ATCC8739) is shown in Figure 3a.
The MICs of AgNPs for each test strain were loaded into the wells formed on plates containing a bacterial lawn.
Following each test, strain recovery data was collected and final residual plastic strain was determined.
If the joint strength is slightly mobilized or immobilized (i.e., most joints keep open) during the test, strain softening occurs.
A single colony of test strain was grown overnight in LB broth medium on a rotary shaker (200 rpm) at 35°C.
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