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As demonstrated on a test sphere, the methodology reduces dominant error sources on the test sphere surface from 194 to 40 nm RMS.
The bed was made using two rows of spheres fixed in staggered positions, and a test sphere resting on top of the three neighbouring fixed spheres (i.e. nestling in the space between the surfaces of the fixed spheres).
A 50 mm-high spillway gate was located downstream of the test sphere in order to obtain deeper water upstream, and provide more easily monitored and controllable water flows.
The influences of a surrounding sphere of different size, located at various distances and angles, on the drag and the flow characteristics of a test sphere have been experimentally investigated.
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The critical flow velocity required to initiate incipient motion in the five test spheres of different dimensions was measured by acoustic Doppler velocimetry.
The free volume fraction for the test spheres with a radius of 2.5 nm is ϕ = 0.695 in the detailed model cell used for the simulations, leading to f volume = 1.44.
In our tests Sphere blog relevance is very good.
The literature expresses large differences in this respect, with tested sphere-to-background ratios ranging from 3 1 [ 19, 31] to 40 1 [ 12], and associated background concentrations from as low as 37 kBq/ml to as high as 470 kBq/ml [ 20].
To test, whether sphere cells could be serially enriched, we seeded 20000 sphere cells over several passages into the SC-medium and determined the number of spheres at each passage (Figure 1B).
To test whether sphere cells of this setting were resistant to cisplatin, we compared sphere formations in culture plates with and without the presence of cisplatin.
We should notice that this range is more limited than that tested for sphere beds.
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