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In this study, an ssDNA library (ssDNA_ODN) that contained a randomized sequence region of 49 nucleotides and two fixed primer hybridization regions was used as a test soiling.
This study demonstrated for the first time the applicability of synthetic ssDNA oligonucleotides as a test soiling on stainless steel surfaces for the validation of medical cleaning processes.
The established qPCR method enables the ultrasensitive and highly specific quantification of the residual synthetic ssDNA test soiling on test objects.
Here, we use for the first time synthetic single-stranded DNA (ssDNA_ODN), which does not appear in nature, as a test soiling to evaluate the cleaning efficiency of routine washing processes.
In this study, ssDNA molecules with a length of 90 nucleotides were used as a test soiling on stainless steel test objects to evaluate the efficiency of medical cleaning processes.
Here, we applied for the first time a synthetic single-stranded DNA (ssDNA) library, which is commonly used as starting material for the selection of DNA aptamers, as a new artificial test soiling on test objects to evaluate the cleaning efficiency of medical cleaning machines.
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