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Sodium and chloride ion contents increased in both root and shoot whereas potassium ion content decreased in shoot of test seedlings with increasing concentrations of NaCl.
Regardless of whether nitrogen was applied, the length of stems, roots, the number of nodes, and thickness of leaves of the test seedlings were inhibited by the presence of Cd in the medium.
For dehydration test, seedlings were transferred to Petri dish covers and left at growth chamber for different length of times.
Inoculation was performed by dusting or brushing conidia from neighboring sporulating susceptible seedlings onto the test seedlings.
Open-pollinated seeds were collected from each wild tree, and test seedlings for each parent tree were grown in nursery beds near Prince George.
Each host species was arranged into ten 36-seedling blocks: 31 test seedlings, each randomly assigned to a Bradyrhizobium strain, and five seedlings that received the sterile water control inoculum.
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In total, 215 SNPs showed a MAF > 0.05 in the sets of tested seedlings.
After 30 d, the fresh weights of 5 to 10 seedlings from each transgenic line and WT plants in each treatment were measured, and the stem lengths of tested seedlings were also measured and compared.
Combined stem and leaf assays can identify more families than can either assay alone, but stem assays are more expensive, requiring controlled growth chambers and the sacrifice of tested seedlings.
Because each tested seedling was genetically related to the mother plant, the statistical analysis of significant differences in bolting rates among populations was carried out, using r version 2.7.1, by means of generalized estimating equations (GEE) to determine parameter estimations for correlated data (Liang and Zeger 1986).
The goals of this study are twofold: (i) to test whether seedlings of E. multisetus plants collected from invaded areas are more successful when grown with B. tectorum than seedlings from nearby uninvaded areas and (ii) to identify what growth traits may help E. multisetus seedlings to establish in B. tectorum-invaded areas.
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