Humans, unlike the test rats, typically ingest minute quantities of phthalates over many years.
So he upgraded the simple cage to a complex one: a maze devised to test rats' navigational skills.
When his test rats couldn't get through their day without an ever-increasing number of nicotine hits — topping out at an astonishing 90 puff equivalents a day — Dr. DeNoble and his employers were equally shocked, though their reactions to the findings were very different.
At test, rats received nonreinforced presentations of LM X and LM Y on separate trials.
Immediately or up to one day after retention test, rats were anesthetized with urethane for LTP experiments.
After the pain threshold test, rats were anesthetized with 10% chloral hydrate solution (300 mg/kg) and then decapitated on the 7th day after surgery.
In one standard test, rats placed in a cylinder of water were considered more depressed if they devoted more time to floating, as opposed to paddling.
He created additional images to present to his test rats by airbrushing the photos so that the rat's face, body, or both were blurred.
The results were counted as the total amount of net ipsilateral rotations per 120 min. One day after the drug-induced rotational test, rats were anesthetized with an overdose of pentobarbital (90 mg/kg, i.p., Mebunat, Orion Oyj, Espoo, Finland).
This choice is also consistent with the recommendation of the OECD that fora chronic toxicity test, rats of the same strain should be used as in studies on thesame substance but of shorter duration [32].
To reduce blood sampling volume before a severe surgical stress and to assess RIFLE score, we measured baseline blood creatinine in ten training test rats and considered the mean creatinine value for the RIFLE calculation.
