Sentence examples for test promoters from inspiring English sources

We distributed a set of test promoters to multiple labs and found good agreement in the reported relative activities of promoters so measured.

To test promoters, we chose the lens crystallin (Emelyanov and Parinov, 2008) and the sonic hedgehog (shh) promoter (Neumann and Nuesslein-Volhard, 2000).

Relative activities of the test promoters in SCBV LUC constructs were compared by normalizing LUC levels to GUS levels as the ratio of LUC/mg protein:GUS/μg protein.

Promoter studies were carried out using a dual luciferase reporter assay (Promega), allowing the analysis of test promoters via firefly luciferase activity and normalization of transfection efficiency via Renilla reniformis luciferase driven by the constitutive CMV-IE promoter in a single-well.

As a test promoter we used the T. gondii ribosomal protein RPS13 promoter for which we provide experimental evidence of having a single major transcriptional start site, a condition favourable to the design of inducible expression systems.

For the self-activation test, promoter bait strains were grown on the SD/−Trp, -His (a synthetic Trp and His dropout medium) media in the presence of 0 mM, 10 mM, 30 mM and 50 mM 3-aminotriazole (3-AT).

To test promoter activity in rice, the OsNCED3 promoter, encompassing 2407 bp upstream of the ATG start codon of OsNCED3, and the OsNCED4 promoter, a total of 1939 bp upstream of the ATG start codon of OsNCED4, were amplified by polymerase chain reaction (PCR) and fused to a β-glucuronidase (GUS) coding region in the pCAMBIA1305.1 binary vector.

The transcription data complement those obtained by fluorescence measurement from the reporter strains, reinforcing the utility of the promoter-probe vectors as a genetic tool to test promoter activity in L. biflexa.

In chi square test, promoter hypermethylation of MGMT was significantly associated with a loss of expression in gastric carcinomas (Table 1-wrap>, P<0.001).

The ability to test promoter sequences for their activity on the PD axis should aid in the investigation of other transcription factors that may be implicated.

A series of broad-host-range vectors carrying a promoterless lacZYA operon was constructed to test promoter activity in a wide range of Gram negative hosts.