Sentence examples for test promoter from inspiring English sources
For the self-activation test, promoter bait strains were grown on the SD/−Trp, -His (a synthetic Trp and His dropout medium) media in the presence of 0 mM, 10 mM, 30 mM and 50 mM 3-aminotriazole (3-AT).
As a test promoter we used the T. gondii ribosomal protein RPS13 promoter for which we provide experimental evidence of having a single major transcriptional start site, a condition favourable to the design of inducible expression systems.
To test promoter activity in rice, the OsNCED3 promoter, encompassing 2407 bp upstream of the ATG start codon of OsNCED3, and the OsNCED4 promoter, a total of 1939 bp upstream of the ATG start codon of OsNCED4, were amplified by polymerase chain reaction (PCR) and fused to a β-glucuronidase (GUS) coding region in the pCAMBIA1305.1 binary vector.
The transcription data complement those obtained by fluorescence measurement from the reporter strains, reinforcing the utility of the promoter-probe vectors as a genetic tool to test promoter activity in L. biflexa.
In chi square test, promoter hypermethylation of MGMT was significantly associated with a loss of expression in gastric carcinomas (Table 1-wrap>, P<0.001).
The ability to test promoter sequences for their activity on the PD axis should aid in the investigation of other transcription factors that may be implicated.
To test promoters, we chose the lens crystallin (Emelyanov and Parinov, 2008) and the sonic hedgehog (shh) promoter (Neumann and Nuesslein-Volhard, 2000).
Relative activities of the test promoters in SCBV LUC constructs were compared by normalizing LUC levels to GUS levels as the ratio of LUC/mg protein:GUS/μg protein.
None of the tested promoter constructs showed an increased reporter activity due to c-MYC.
When compared to Nter-15Q, Nter-142Q led to an increase of luciferase activity for all tested promoter regions, with the exception of region A1.
Surprisingly, the expression of the c-MYC protein led to an inhibition of all tested promoter constructs independent of the selected serum concentrations.
