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Biodegradable composite ('biocomposite') test plaques consisting of Miscanthus fibres in a Novamont Mater-Bi® matrix were produced and tested in the laboratory.
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The recovered virus had the same in vitro characterization as the parental virus with regard to antigenicity in neutralization and indirect immunofluorescence tests, plaque size and one-step growth.
EEEV-positive pools were directly tested and quantified by using 2 independent tests (plaque titration and qRT-PCR).
For each MS sample, two areas were tested: MS plaques and normal cervical spinal cord, for a total of 24 samples.
Spinal fluid tests of plaque and tangle proteins, like a Tau/Abeta ratio greater than 0.39, have a relatively high accuracy for Alzheimer's but are not 100 percent accurate.
Every 10 passages, the culture supernatant was collected and tested by plaque reduction assay.
The inhibitors tested decrease plaque size for VV, ectromelia virus and monkeypox virus, implying that PI3K-dependent mechanisms are conserved among poxviruses.
To assist in interpreting pool water virus titers, we conducted an in vitro experiment in which pool water from a room containing non-infected ducks was spiked with the H5 or H7 viruses, maintained at room temperature and tested by plaque assay, as described above, at intervals up to 42 days.
These mixtures were then tested by plaque assay.
Sera were tested by plaque neutralization (PN) and microneutralization (MN) assays.
Virus neutralization activity of mouse sera was tested by plaque reduction assay.
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