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In the analysis of the prostate cancer metastasis dataset, we aimed to compare the effectiveness of MPINet and other currently popular methods, including the hypergeometric test (ORA), MSEA (SEA), and MetPA.
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The Blast2GO program utilizes a Fisher's exact test for ORA and corrects the p-value to control the increased false discovery rate (FDR) associated with multiple hypotheses testing.
For the Pageman analysis, Fisher's test for ORA (over-representation analysis) analysis was carried out in Pageman in order to determine statistically significant over/under representation of genes classified into specific BINS.
After ensuring model adequacy using the Hosmer Lemeshow test, adjusted odds ratios (ORa) were calculated.
With this data set we performed an Over Representation Analysis (ORA) to test whether among the 258 differentially expressed genes specific biological categories or pathways were affected.
Based on these first efforts, Liu et al. (2007) have proposed Gene Network Enrichment Analysis (GNEA) that uses ORA to test for overrepresentation of gene sets in transcriptionally affected subnetworks of a global interaction network.
We determined the effectiveness of MPINet for identifying pathways associated with prostate cancer metastasis and compared the results with those of three other popular methods, including ORA (hypergeometric test), MSEA, and MetPA.
Together with all other ophthalmic examinations, indices gained from ORA were tested between 7 a.m. to 10 a.m., which largely avoided the diurnal fluctuation mentioned by the manufacture [ 2].
O2EM allows over-representation analyses (ORA) based on Fisher's exact test [ 33] to validate the significance of the expression status for each protein family separately and for each organism.
The Kolmogorov Smironov test was used to check for normality of the ORA BSV measurements.
Over- and Under-Representation Analysis (ORA) of functional categories [ 34] in the test set (a set of differentially expressed genes) was done using GeneTrail (http://genetrail.bioinf.uni-sb.de) and a reference set comprising all gene IDs of Arabidopsis ATH1 GeneChip.
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