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The Approximately Unbiased (AU) test of tree topologies [ 22] was applied using TreeFinder [ 32].
*Number of incongruent genes at the p<0.05 level based on a Shimodaira-Hasegawa (SH) test of tree topologies.
1. Placing the bacterial branches outside the eukaryotic tubulin subtree was firmly rejected by the same statistical test of tree topology that we did in the paper (AU < 0.01).
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Statistical tests of tree topologies and probabilistic counts of gene duplications were performed using CONSEL version 0.20 [ 26], as previously reported [ 11].
Using these sequences we have performed genome mapping, molecular phylogenetic analyses using Maximum Likelihood and Bayesian methods, and statistical tests of tree topologies, to ascertain the phylogenetic relationship among ependymin proteins.
In contrast to the study of Lemieux et al. [ 13] in which both T2 and T3 proved to be significantly worse than T1 in confidence tests of tree selections, only the T3 topology was rejected at the 5%percentt confidence level (T2, P = 0.135; T3, P = 0.031) in Approximately Unbiased (AU) tests.
Because we cannot know the ground truth for real data with certainty, we used simulated trees to test accuracy of tree inferences.
Constrained trees were generated manually and evaluated according to the Shimodaira/Hasegawa test of alternative tree topologies [ 97] as implemented in TREE-PUZZLE.
Bootstrapping tests of trees were performed using 100 sampling repetitions.
All areas where the fungus has been found remain open to the public.[2] Testing of trees and soil is being done in a number of areas on Vancouver Island and in the Vancouver Coastal and Fraser Health Regions to help determine the general distribution of the fungus.
The AU test of phylogenetic tree selection [ 53], showed that the original unconstrained tree (groups C. parapsilosis with proteobacteria) receives the optimal likelihood tree score, and the differences in likelihood scores when compared to the constrained trees [see additional file 6], are significant (P < 0.05).
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