All preliminary validation steps have been done: standardization of all assays, reproducibility of the qPCRs in parallel and in independent runs, selection of reference samples and testing of reference genes http://www.gene-quantification.info/.info/
For each subject, the test order of reference orientations and meridian locations at each eccentricity was pseudo-randomly assigned.
As possible sources of heterogeneity, we explored: geographical area, commercial brand of index test, type of reference standard, disease prevalence, study size, and risk of bias (QUADAS-2).
After their completion of the test, members of reference panel were asked to identify the items they found confusing or not relevant.
In order to facilitate the presentation, these methods are defined as "reference methods" in the following text, and the test results of reference methods are called "reference results".
The log2ratio of test to reference was calculated and plotted according to chromosomal position of the clones.
A number of isothermal nucleic acid amplification techniques have been developed to enable performance of nucleic acid testing outside of reference laboratories.
M indT heG ap detects insertion sites by scanning the reference genome and testing membership of reference k-mers in the de Bruijn graph.
The copy number of the test locus is then estimated from the ratio of test to reference products.
The basic design principles of the two systems are presented and the systems are tested using a multitude of reference test benches.
