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This software was also used for Mantel's test of isolation by distance.
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A test of isolation-by-distance was carried out by applying a Mantel's test to matrices of genetic and geographic distances in G enA lE x using 999 permutations.
Mantel tests of isolation by distance for population pairs found no significant correlations between geographic distance and FST/ 1-FST) (P = 0.410; FST/ 1-FST
This was further verified with Mantel tests of isolation by precipitation (IBP).
Tests of isolation by distance were performed separately for all of the populations together, for each population cluster, and for all of Amazonia.
Mantel tests of isolation by distance (IBD) were evaluated in genalex v.6.2 using matrices of pairwise FST and pairwise stream distance.
Other staff outcomes included: gains in knowledge (tested via knowledge tests), feelings of isolation or socialisation (for example, connectedness with other centres or colleagues), levels of information exchange and networking, and retention (intention to leave and staff turnover).
Statistical tests of isolation-by-distance were carried out for each gene among all populations and by studying populations from reference and polluted sites separately.
Further investigations (such as common garden experiments) are required for rigorous testing of isolation-by-adaptation in order to understand how local adaptation and geographic isolation interactively affect the pattern of genetic differentiation within a plant species.
Correlation coefficients of Mantel tests of isolation-by-distance were r = 0.297 (P < 0.001) and r = 0.089 (P < 0.001) for all spotted owls and just northern spotted owls, respectively.
For both nrDNA and cpDNA data, tests of isolation-by-distance (IBD) were performed by regressing values of ΦST against the geographical distance (Km) and the ecological distance (least cost distance) with the Mantel permutation procedure as implemented in IBD (Isolation by Distance Web Service: BMC Genetics 6: 13. v.3.16 http://ibdws.sdsu.edu/) [ 59].
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