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Monogenic test of inheritance showed that resistance to pyriproxyfen was governed by multiple genes.
A more formal quantitative genetic test of inheritance was performed by indexing each of the 16 mother-newborn pairs with a parameter indicating their familial relationship.
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We used the results from PennCNV for our study since it showed better compliance with the test of Mendelian inheritance.
Although this test is usually used to test for independence of inheritance of supposedly physically unlinked genetic markers, we used it to test the degree of correlation between proximate regions within the rDNA ITS locus (<300 base pairs apart).
The primers were designed to enable testing for inheritance of the at risk HD allele using two multiplexes, with each marker designed to amplify a different PCR product size and assigned a tag (6FAM™, VIC®, NED™ or PET™ dyes; Applied Biosystems) to ensure no overlap in marker fragments.
Haplous is flexible and allows various testing of various inheritance models and datasets such as a recessive family-based inheritance model, IBD analyses in multiple pedigrees of any size, homogenous population samples, or any combination of these.
Indeed, we were able to test the hypothesis of "inheritance from the rare species" because we observed an alternation of gene flow between the two species in the same river, and because we knew that hybridization was contemporary, making it possible to "calibrate" each of the two species in allopatric zones.
The segregation analysis, as implemented in the statistical package MAN (Malkin and Ginsburg 2003), was utilized in this study to test a mode of inheritance of the skeletal biomarker of BA TOSS.
This allowed for maximal power in testing of Mendelian inheritance across markers.
We had 80% power to detect a hazard ratio of 1.57 under the tested recessive model of inheritance, given our sample size of 704 participants and a minor allele frequency of 17%.
We tested the frequency of inheritance of VAND vs. ME49 chromosomes and found that it did not differ significantly from the expected 50/50 ratio under the assumption of random segregation.
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