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Individuals from the six species-pairs with adequate samples sizes were picked randomly to query whether they could be assigned correctly to their species using clustering in a NJ tree, fixed mutations, and a statistical test of assignment based on coalescent theory [ 24] (Table 2, Figure 2).
For this purpose, a total of 750 tissue samples from Chinook salmon of known life history stored in the CDFG tissue archive were coded (to mask their identity) and enabled a blind test of assignment accuracy of three alternate microsatellite panels.
To further investigate the influence of this factor, we have performed two different tests: a deletion experiment, in which a percentage of the database is removed to simulate a reduction in our current knowledge, and a test of assignment involving sequences from rare taxa, which probably represent the most serious challenge to the method.
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The tests of assignment in a Bayesian statistical framework resulted in correct assignment to species and subspecies in all instances (Table 2).
Given the number of loci used, only first-generation immigrants would be detectable (see Rannala and Mountain 1997 for a discussion on the power of statistical tests of assignment).
We also applied Canonical Variates Analysis (CVA) in CVAGen6 [76], [76] to determine if we could assign specimens to morphotype based purely on the measured shape of the specimens when considering all morphotypes simultaneously, and we estimated the rate of correct assignments with a jackknife-test of assignments based on the CVA axes.
This approach on dispersal was complemented by tests of assignments.
Table 1 shows the numbers of rats and the test group assignment of all of the animals.
Our empirical test of higher taxonomic assignments reveals that a tree-based assignment system would successfully assign most queries to a higher taxon at some level.
We further designed a set of experiments to test the assignment of miR-200a as the top regulator of a small module of nine genes.
An important assumption of assignment test models is that populations are in Hardy-Weinberg and linkage equilibrium or nearly so [ 29, 55, 56].
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