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In some cases, he and others said, it is not necessary to test genes.
To satisfy sample size requirement of normal approximation by chi-square test, genes with expected values less than 5 were removed.
Expression levels of test genes were normalized to expression levels of gyrase (gyr) message.
First, we test genes for their orthology against the other environment.
All the test genes were expressed relative to a control gene, β-actin or GAPDH.
The value for one of the test genes was highly positive (the msp3/6-like gene PF10_0348).
This analysis identified the teams that predicted well (over the 50 test genes) at each time point.
Growth for several cell cycles in the presence of the HDAC inhibitor Trichostatin A (TSA) induced hyperacetylation and transcription in normally silent test genes inserted into centric heterochromatin.
The final normalized results were calculated by dividing the relative transcript levels of the test genes by the relative amount of the β-actin RNA.
The p-values (based on Spearman correlation coefficient) computed over the set of 50 test genes at each of the eight time-points are reported in Table 6.
The fluorescence signals of the 28 pathogenic samples, including 50 of 57 test genes, were much stronger than those of the negative controls (Figure 5).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com