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To test for preference of one prey type, log ratios (LR) were calculated as described by Roy et al. (2008): where i is the number of consumed infected larvae and u is the number of consumed uninfected larvae.
The experimental mouse was placed in the center chamber that was open to both sides and allowed to explore for 5 min. To test for preference for social novelty, the experimental mouse was again placed in a holding cage while a different unfamiliar mouse with the characteristics described above was placed under the empty woven metal container.
In the test for preference of social novelty and social recognition, the 129SVE mice failed to show a significant preference for the unfamiliar mouse compared with the familiar mouse; however, a trend was observed in knockout mice, which spent more time with the unfamiliar mouse than with the familiar mouse (129SVE: WT: t18=−1.30, P=0.210; Het, t22=−1.57, P=0.130; KO: t11=−1.99, P=0.071).
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To test for preferences for one side of the room that may influence the results, bees were also tested in this fashion using two identical ambient temperature feeders at 28°C and found to have no preference; choice for the 'Eastern' feeder was 50.3%+/− 2.0 which was not significantly different to chance expectation (1 sample t-test, t = 0.466, df11, p = 0.650).
Extensive preliminary tests for preference of cage side, saccharin preference over water, saccharin concentration and one- versus two-bottle paradigm conducted before the CMS period in the long-term CMS paradigm resulted in the following measurement protocol.
These strains were tested for preference behavior as homozygotes.
Recombinants resulting from two or three iterative rounds of recombination [(CSSIV × N2) × N2 (× N2)] were genotyped across chromosome IV and tested for preference behavior as homozygotes.
Because we found differences between populations of weevils sampled from different hosts, we also tested for preference differences between weevils that could use different hosts in their field site.
Conditioned ethanol preference is measured by presenting two odors in sequence, one in the presence of an intoxicating dose of ethanol, three times for 10 min with 50-min breaks between exposures, followed by testing for preference between the two odors in a Y-maze in the absence of ethanol either 30 min or 24 hr after training.
At the end of the selection process, we separately introduced each morph type (black and brown) to a mixture of resources, to test for resource preference.
In cases in which individuals performed more than 6 actions at the feeding box, we used a binomial test to test for a preference for one technique.
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