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An earlier study investigated NIR hyperspectral imaging as a tool for high-throughput analysis of self-contained microbial identification of test cards for microorganisms of concern in food [ 37].
Chapter 2.6.12 Microbiological examination of non sterile products: Microbial enumeration tests and Chapter 2.6.13 Microbiological examination of non sterile products: Test for specified microorganisms.
The identification tests used for microorganisms have been previously described [ 6].
Both the forensiX and the Sarstedt swabs were tested for viable microorganisms after 14 days of storage to investigate whether drying the saliva samples preserved the DNA by killing the associated microorganisms or by suspending their metabolism.
Similarly, the next 2 groups (33 samples each) were tested for the microorganism at Day 3 and Day 7 after the purchase date and stored in refrigerator at 4°C.
Ciprofloxacin (5 μg/well) for bacteria, fluconazole (5 μg/well) for fungi were used as positive control and DMSO was used as negative control for test microorganisms.
The optimised reactor design achieved S90 values (cumulative exposure required for 90% reduction) for the test microorganisms in the range 0.63 1.82 MJ m−2 of Global Solar Exposure (GSX) without the need for TiO2 as a catalyst.
However, a number of studies have reported low sensitivities of tissue sampling, [ 9– 11] and a more reliable test for identifying the correct microorganism is desirable.
Further research could test for the development of microorganism on corpses in the nest, and the consequences on the immune system activity, investigating the costs and benefits of necrophoresis.
The most effective disinfection method was 100% white vinegar for tested microorganisms and both acrylic resins.
This study showed that white vinegar 100% was the most effective method for tested microorganisms.
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