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A Mantel test as implemented in Mantel Nonparametric Test Calculator, version 2.0 [ 110] was used to test for isolation by distance within identified lineages/haplogroups.
GENEPOP 4.0 [38], [38] was used to test for isolation by distance using a Mantel test.
A test for isolation by distance was performed by regressing ΦST/1- ΦST against the natural logarithm of geographical distance using GENEPOP, version 3.4 [3.4.
Mantel tests [41] were conducted in GENEPOP to test for isolation by distance, using the natural logarithm of geographic distance and linearized estimates of FST/ 1-FST) FST/ 1-FSTopulation pair.
We also plotted linearized pairwise values of both FST and RST against log-transformed geographic distances (km) to test for isolation by distance (IBD), using a Mantel test with permutations (1000 times) to assess significance in GENEPOP.
A correlative test for isolation by distance was performed with pairwise K2P genetic distance (the same distance calculated by Arlequin for the Fst analysis) and effective geographic distances estimates among sites.
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The mantel test for isolation-by-distance (IBD) established relationships between genetic and geographic distances [ 40] and genetic distances (FST / (1 –FST)) were regressed against the logarithm of geographic distances within location distances (estimated with Google Earth 6.0).
A Mantel test [ 16] was performed using the statistical software R version 2.15.0 [ 17] to test for isolation-by-distance, by investigating the correlation that exists between genetic and geographical distances between pairs of breeds.
A Mantel test of matrix correspondence (Mantel 1967) in GenAlEx version 6.1 (Peakall and Smouse 2006) was used to test for isolation-by-distance following methods of Smouse et al. (1986), by examining the correlation between matrices of Nei's genetic distance calculated according to methods outlined in Lynch and Milligan (1994) (AFLP-SURV 1.0) and geographic distance.
Mantel tests for isolation by distance analysis were calculated with ISOLDE as implemented in GENEPOP [20].
We tested for isolation by distance by comparing genetic against geographical distances using Mantel tests.
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