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Despite its importance, there is no universal test for disequilibrium (Slatkin, 2008).
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GenAlEx 6.5 offers pairwise tests for disequilibrium between biallelic markers such as SNPs.
None of the pairs of loci showed significant linkage disequilibrium (all P > 0.4, exact test for genotypic disequilibrium), and summary statistics for all 10 loci are given in Table S1.
For each of the 8 loci, we tested for Linkage Disequilibrium (Gamete Disequilibrium Test), for deviations from Hardy-Weinberg Equilibrium (exact HW test) and for heterozygote deficiency (expected if null alleles are present, U test) in a sample of 64 non-relatives from our study, using the software Genepop 4.0 [27].
In addition, the linkage disequilibrium (LD) option in PROC HAPLOTYPE was specified which generated a likelihood ratio test for linkage disequilibrium testing a null hypothesis of no association between the SNPs within a given haplotype region.
We used the Helix Tree software (Golden Helix, Inc., Bozeman, MT, USA; http://www.goldenhelix.com/pharmhelixtreefeatures.html) to test for linkage disequilibrium among the 46 SNPs; 6 SNPs were shown not to be in linkage disequilibrium.
However, we did not test for linkage disequilibrium in this study.
We also used FSTAT to test for linkage disequilibrium (LD) between loci.
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