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The exact test for departure from HWE was also performed using FSTAT.
We examined the issue of linearity by performing a test for departure from linear trend which was not significant.
Tajima's D uses the average number of pairwise differences and number of segregating sites in the intraspecific DNA sequence to test for departure from neutral expectations, generally assuming negative values in populations that have experienced size changes, or for sequences that have undergone selection.
The same program was used to test for departure from HWE for the polyploid population.
However an alternative approach is to test for departure from HWE in cases.
A Chi-square goodness-of-fit test for departure from expected values yields P = 0.13.
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Both Fu's FS and Tajima's D use the infinite site-model without recombination to test for departures from selective neutrality and population equilibrium for intraspecific data.
Second, we utilized the X2 goodness of fit test with continuity correction 0.25 to test for departures from Hardy-Weinberg-Equilibrium Hardy-Weinberg-Equilibrium Hardy-Weinberg-Equilibriumts using tHWEr published genotypes amonge locontrolsterest.
We calculated several classical population genetics statistics to further test for departures from equilibrium conditions.
Population summary statistics examine genetic variability within populations and can be used to test for departures from demographic equilibrium.
In addition, the Hudson Kreitman, and Aguade (HKA) test (Hudson et al. 1987) was used to test for departures from a neutral model.
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